Home >> Stem Cells & Wnt >> Wnt/beta-catenin >> Wnt/beta-catenin Antibodies >> 

WIF1 Antibody (1G5)

Mouse Monoclonal Antibody
Size Price Quantity
100μl $ 250
Buy and order WIF1 Antibody (1G5)(Anti WIF1 Antibody (1G5)) at reasonable price from reliable supplier Selleck!

Application Data

  • Western blot analysis using WIF1 mouse mAb against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.
  • Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using WIF1 mouse mAb with DAB staining.
  • Immunofluorescence analysis of Hela cells using WIF1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Product Information

Clonality: Mouse Monoclonal Antibody
Isotype: Mouse IgG1
Clone Number: 1G5
Reactivity: Human
Molecular Weight: 42 kDa
Swiss-Prot:

Q9Y5W5
Sensitivity: WIF1 Mouse mAb detects endogenous levels of WIF1 protein.
Immunogen:

Purified recombinant fragment of human WIF1 expressed in E. coli.
Storage: Supplied in PBS, 0.005% sodium azide, PH 7.4. Store at -20°C. Stable for one year from the date of shipment.
Synonyms: WIF antibody; WIF-1wnt inhibitory factor 1 antibody

Tested Applications

Applications Recommended Dilutions Protocols
Western blot 1:500 - 1:2000 Western blot
Immunohistochemistry 1:200 - 1:1000 Immunohistochemistry
Immunofluorescence 1:200 - 1:1000 Immunofluorescence
Enzyme-linked Immunosorbent assay 1:10000 Enzyme-linked Immunosorbent assay
Unconditional Return & Replacement Policy
Free Delivery on All Orders over $500
Overnight Delivery You Can Trust

Background

WIF1 (Wnt inhibitory factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity [1]. It contains an N-terminal WIF domain and five EGF-like repeats [2]. The WIF1 ortholog in Drosophila, Shifted, is required for Hedgehog stability and diffusion [3,4]. It has been reported that WIF1 expression is downregulated in many types of cancers [5-8].