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| Clonality: | Mouse Monoclonal Antibody |
|---|---|
| Isotype: | Mouse IgG1 |
| Clone Number: | 6G9 |
| Reactivity: | Human |
| Molecular Weight: | 81 kDa |
| Swiss-Prot: | |
| Sensitivity: | VCAM1 Mouse mAb detects endogenous levels of VCAM1 protein. |
| Immunogen: | Purified recombinant fragment of human VCAM1 expressed in E. coli. |
| Storage: | Supplied in PBS, 0.005% sodium azide, PH 7.4. Store at -20°C. Stable for one year from the date of shipment. |
| Synonyms: | CD106 antibody; DKFZp779G2333 antibody; INCAM-100 antibody; MGC99561 antibody; CD106 antigenvascular cell adhesion protein 1 antibody; vascular cell adhesion molecule 1 antibody |
| Applications | Recommended Dilutions | Protocols |
|---|---|---|
| Western blot | 1:500 - 1:2000 | Western blot |
| Immunohistochemistry | 1:200 - 1:1000 | Immunohistochemistry |
| Enzyme-linked Immunosorbent assay | 1:10000 | Enzyme-linked Immunosorbent assay |
Human VCAM1 (CD106), a member of the immunoglobulin superfamily, is a cell surface sialoglycoprotein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM1 expression is induced by IL1β, IL4, TNFα and IFNγ. VCAM1 binds to leukocyte integrins VLA4 and α4β7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM1, on endothelium. The VCAM1: VLA4/α4β7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. ELISA techniques have shown that detectable levels of soluble VCAM1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM1 may be elevated or lowered in subjects with a variety of pathological conditions.
