- |
My Account- |
United States (Change Country)You can order by phone, email or fax Tel: +1-832-582-8158 Fax: +1-713-796-9816 Email: [email protected] |
| Formulation: | A collection of 1484 bioactive compounds supplied as lyophilized powder or pre-dissolved DMSO solutions. | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Container: | 96 Well Format Sample Storage Tube With Screw Cap and Optional 2D Barcode | ||||||||||||
| Stability: |
|
||||||||||||
| Shipping: | Blue ice | ||||||||||||
| Packaging: | Inert gas |
| We treated all of drugs in T47D which has a PI3KCA H1044R mutation with the concentration shown below for 1 hour and performed western blot analysis using antibodies to phospho-AKT(SERINE 472), and total AKT. |
Data were provided by Saraswati Sukumar of Johns Hopkins University School of Medicine.
AS-605240,GDC-0941,GSK1059615,LY294002,PI-103,PIK-90,TG100-115,TGX-221,ZSTK474 were supplied by Selleck
We treated all of drugs in T47D which has a PI3KCA H1044R mutation with the concentration shown below for 1 hour and performed western blot analysis using antibodies to phospho-AKT(SERINE 472), and total AKT.
| (A) VimPro-Fluc activity in spheroids after 72-h treatment with control modulators of epithelial-mesenchymal transition (EMT) normalized to spheroid viability and compared to vimentin protein expression using Western blot analysis. (B) Dose-response curves for both U0126 and axitinib control modulators of EMT. RLU, relative luminescence units. |
Data from Journal of Biomolecular Screening 16(2)
Axitinib and PF-2341066 were purchased from Selleck
(A) VimPro-Fluc activity in spheroids after 72-h treatment with control modulators of epithelial-mesenchymal transition (EMT) normalized to spheroid viability and compared to vimentin protein expression using Western blot analysis. (B) Dose-response curves for both U0126 and axitinib control modulators of EMT. RLU, relative luminescence units.
| Secondary assay development. The invasive potential of MDA-MB-231 spheroids was measured using modified Boyden chambers coated with Matrigel™. Invading cells were fixed, stained with DAPI, and quantified by fluorescence microscopy using 5 random fields per filter insert in triplicate. U0126, PF2341066, axitinib, and PKC412 inhibited the invasive potential of MDA-MB-231 spheroids by ~90% as compared to untreated spheroids (UT). ***p ≤ 0.001. IGF1R and dasatinib displayed no statistical difference as compared to UT MDA-MB-231 spheroids. |
Data from Journal of Biomolecular Screening 16(2)
Axitinib and PF-2341066 were purchased from Selleck
Secondary assay development. The invasive potential of MDA-MB-231 spheroids was measured using modified Boyden chambers coated with Matrigel™. Invading cells were fixed, stained with DAPI, and quantified by fluorescence microscopy using 5 random fields per filter insert in triplicate. U0126, PF2341066, axitinib, and PKC412 inhibited the invasive potential of MDA-MB-231 spheroids by ~90% as compared to untreated spheroids (UT). ***p ≤ 0.001. IGF1R and dasatinib displayed no statistical difference as compared to UT MDA-MB-231 spheroids.
| Inhibition of anchorage-independent growth of lung tumor cell lines by selected inhibitors. Each selected cell line was treated with the indicated inhibitor at 0.1 μM and 1 μM concentrations for two weeks and cell colony size formation was scored under the Nikon inverted-phase microscope. |
Data from International Journal of Proteomics Volume 2011, 13 pages
BIBW-2992, Erlotinib, BMS-536924, PD-0325901 and PF-2341066 were purchased from Selleck
Inhibition of anchorage-independent growth of lung tumor cell lines by selected inhibitors. Each selected cell line was treated with the indicated inhibitor at 0.1 μM and 1 μM concentrations for two weeks and cell colony size formation was scored under the Nikon inverted-phase microscope.
| Ba/F3 cell lines expressing the recombinant TEL/kinase domain fusion protein for FGFR1-4 .Cells were grown in RPMI 1640 containing 10% FBS and 500 ng/mL puromycin. The parental Ba/F3 cell line transduced with an empty vector was grown in 10 ng/mL IL-3 (R & D systems). Cell viability was assessed at 72 hours using the Cell Titer 96 Aqueous One Solution (Promega). Data were plotted as percent viability relative to vehicle-treated cells and are shown as mean (± SD) from 3 experiments. |
Data from Blood 2010;116:210.
Cediranib was purchased from Selleck
Ba/F3 cell lines expressing the recombinant TEL/kinase domain fusion protein for FGFR1-4 .Cells were grown in RPMI 1640 containing 10% FBS and 500 ng/mL puromycin. The parental Ba/F3 cell line transduced with an empty vector was grown in 10 ng/mL IL-3 (R & D systems). Cell viability was assessed at 72 hours using the Cell Titer 96 Aqueous One Solution (Promega). Data were plotted as percent viability relative to vehicle-treated cells and are shown as mean (± SD) from 3 experiments.
A collection of 729 inhibitors
A collection of 118 tyrosine kinase inhibitors
A collection of 277 kinase inhibitors
A collection of 789 FDA approved drugs
A collection of 146 natural products
A collection of 84 chemotherapeutic agents
