Transforming Growth Factor β (TGFβ) can induce anchorage-independent growth, as a founding member of the largest family of secreted morphogens in mammals. There are four subclasses of the TGFβ family with different biological functions including activins, inhibins, bone morphogenetic proteins (BMPs), and Mullerian-inhibiting substance (MIS). After transmembrane serine/threonine kinases binding form a heteromeric complex of type II and type I receptors, TGFβ signaling pathway is activated. Smad signaling pathway is activated after the phosphorylation of the type I receptor by the type II kinase. In the eponymous TGFβ pathway this occurs via ligand-induced cooperative assembly of the receptor complex, thus allowing the kinase domain of the type II receptor to phosphorylate the type I receptor, as well as other receptor-bound components. In the canonical pathway, signaling to the transcriptional achinery is transduced by a unique family of intracellular signaling mediators called Smads. There are so many important targets related to Smad including HDAC1, Notch, STAT3, ERK and p38.