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Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB staining using CHK2 mouse mAb.
| Clonality: | Mouse Monoclonal Antibody |
|---|---|
| Isotype: | Mouse IgG2b |
| Clone Number: | 1C12B8 |
| Reactivity: | Human |
| Molecular Weight: | 61 kDa |
| Swiss-Prot: | |
| Sensitivity: | CHK2 Mouse mAb detects endogenous levels of CHK2 protein. |
| Immunogen: | Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. coli. |
| Storage: | Supplied in PBS, 0.005% sodium azide, PH 7.4. Store at -20°C. Stable for one year from the date of shipment. |
| Synonyms: | CHEK2 antibody; CHK2 checkpoint homolog (S. pombe) antibody; serine/threonine-protein kinase Chk2 antibody; protein kinase CHK2 antibody; checkpoint-like protein CHK2 antibody; CDS1 antibody; HuCds1 antibody; LFS2 antibody; PP1425 antibody; |
| Applications | Recommended Dilutions | Protocols |
|---|---|---|
| Western blot | 1:500 - 1:2000 | Western blot |
| Immunohistochemistry | 1:200 - 1:1000 | Immunohistochemistry |
| Immunofluorescence | 1:200 - 1:1000 | Immunofluorescence |
| Enzyme-linked Immunosorbent assay | 1:10000 | Enzyme-linked Immunosorbent assay |
Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases [1-3]. The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases [4,5]. After DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR [5-7]. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain [8].
