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| Clonality: | Mouse Monoclonal Antibody |
|---|---|
| Isotype: | Mouse IgG1 |
| Clone Number: | 4E3 |
| Reactivity: | Human |
| Molecular Weight: | 78 kDa |
| Swiss-Prot: | |
| Sensitivity: | Bip Mouse mAb detects endogenous levels of Bip protein. |
| Immunogen: | Purified recombinant fragment of human HSPA5 expressed in E. coli. |
| Storage: | Supplied in PBS, 0.005% sodium azide, PH 7.4. Store at -20°C. Stable for one year from the date of shipment. |
| Synonyms: | HSPA5 antibody; GRP78 antibody; MIF2 antibody; 78 kDa glucose-regulated proteinendoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody; immunoglobulin heavy chain-binding protein antibody; heat shock 70kDa prote |
| Applications | Recommended Dilutions | Protocols |
|---|---|---|
| Western blot | 1:500 - 1:2000 | Western blot |
| Immunohistochemistry | 1:200 - 1:1000 | Immunohistochemistry |
| Enzyme-linked Immunosorbent assay | 1:10000 | Enzyme-linked Immunosorbent assay |
Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells [1,2]. It was also found to be induced at the protein level by glucose starvation [3]. When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding [4].
