Lysine-specific demethylase 1 (LSD1), also known as AOF2 and BHC110, is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor . Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes [2,3]. In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 [4,5]. LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells [1,6,7]. In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation . Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine .
Purified recombinant fragment of human LSD1 expressed in E. coli.
LSD1 Mouse mAb detects endogenous levels of LSD1 protein.
Supplied in PBS, 0.005% sodium azide, PH 7.4. Store at -20°C. Stable for one year from the date of shipment.
Human, Mouse, Monkey
Use an anti-MOUSE secondary antibody to detect the antibody.
|Western blot||1:500 - 1:2000|
|Immunohistochemistry||1:200 - 1:1000|
|Enzyme-linked Immunosorbent assay||1:10000|
|Not yet tested in other applications.|
Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and kidney carcinoma (right), showing nuclear localization using LSD1 mouse mAb with DAB staining.